Application

Research CategoryApoptosis & Cancer

Detect Fas Ligand using this Anti-Fas Ligand Antibody validated for use in ELISA & WB.

Research Sub CategoryApoptosis - Additional

Western blotting:1:1000 - 1:5,000, using Chemiluminescent detection. The use of 0.1 - 0.5% milk in diluents is recommended to reduce background. Based upon the calculated amino acid sequences, rat (278), mouse (279), and human (281) FASL has a molecular weight of ~32 kDa. A 40 kDa FASL band has been detected in mouse T cells.

ELISA: 1:10,000 - 1:50,000, for detection of control peptide (AG626) coated at a 1 µg/mL concentration.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

An 18 amino acid peptide sequence (aa 130-146) from rat FASL. This sequence is known to be 94% homologous with mouse FASL, differing by one amino acid. This peptide region does not show significant homology with human FASL, with only four amin

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid antiserum containing 0.05% sodium azide as a preservative.

Storage and Stability

Store at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze-thaw cycles.